In Living Color  Protocols in Flow Cytometry and Cell Sorting

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Table of Contents
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Rochelle Diamond

Susan DeMaggio

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Welcome to the Official Website of
In Living Color
Protocols in Flow Cytometry and Cell Sorting
Rochelle Diamond and Susan DeMaggio, Editors


SUBJECT INDEX

buffer, pH standards
"drop and drag"
"hemostat and lens tissue"
2,3-dicyano-1,4-hydroquinone diacetate (ADB)
5-chloromethylfluorescein di-b-galactopyranoside (CMFDG)
7-AAD
7-aminoactinomycin D (7-AAD)
Absolute cell concentration
acites
acridine orange (AO)
adjuvant
Aequorea victoria
aequorin
aerosol containment
aerosols
agarose
agonist
Algae
alignment, optical
aminopropyltriethoxysilane
amplification
amplification mode
amplification, logarithmic
analog delay line
analog-to-digital converter (A-D)
analysis, 1 & 2 color
analysis, chemical
analysis, immunophenotypic
analysis, multicolor
analysis, trivariate
Annexin V
Annexin-V-FITC
anode
anthocyanins
antibodies
antibodies, nonspecific binding
antibodies, primary
antibodies, secondary
antibodies, titered
antibody
antibody conjugates, direct
antibody conjugates, indirect
antibody, primary
antibody, secondary
Antifade
anti-Fas antibody
antigens
anti-transferrin antibody
APC
APC-CY7 (ALLO-7)
apoptosis
aquatic biology
Arc lamps
area number
Asymmetric cytokinesis
AT:GC ratios
Autoclone
autofluorescence
avetin anesthetic
avidin-magnetic bead
azide
background
background fluorescence
background reduction
bacteria, heterotrophic 
base specificity
baseline restoration
B-cell Harvest
beam dimensions
b-galactosidase (b-gal)
b-geo
bicistronic expression vector
Biohazards
biosafety
biotin
biotin succinimide
biotinylation
bleach
blocking
blocking antibody
blocking solution
blocks, sample and sorter
blue fluorescent protein
boolean gate logic
bovine serum albumin
Brefeldin A
bromodeoxyuridine
Budgeting
calcium flux
calcium ionophore (CaI)
calibration
calibration curve
calibration, fluorescence ratio 
calibration, instrument 
calibration, ratio signal
carboxyseminaphthorhodafluor-1 (c-SNARF-1)
carboxy-SNARF-1-AM
carcinogens
catcher tube
cathode
CCD camera
Cell Census Plus tm System, amplification/compensation
Cell Census Plus tm System, cell proliferation
Cell Census Plus tm System, Cell proliferation model
Cell Census Plus tm System, Cell proliferaton model
Cell Census Plus tm System, cellular proliferation index
Cell Census Plus tm System, color compensation
Cell Census Plus tm System, data analysis
Cell Census Plus tm System, daughter cells
Cell Census Plus tm System, dye dilution
Cell Census Plus tm System, fluorescence amplification
Cell Census Plus tm System, gaussian distributions
Cell Census Plus tm System, low frequency populations
Cell Census Plus tm System, low frequency respones
Cell Census Plus tm System, lymphocytes
Cell Census Plus tm System, mathematical model
Cell Census Plus tm System, mitogen
Cell Census Plus tm System, mixed lymphocyte culture
Cell Census Plus tm System, Modfit Proliferation Analysis
Cell Census Plus tm System, non-proliferating fraction
Cell Census Plus tm System, PKH 26 dye dilution
Cell Census Plus tm System, PKH26
Cell Census Plus tm System, proliferation analysis software
Cell Census Plus tm System, proliferation index
Cell Census Plus tm System, Proliferation Wizard
Cell Census Plus tm System, splenocytes
Cell Census Plus tm System, subset proliferation
Cell Census Plus tm System, subset specific responses
cell contact inhibition assay 
cell cycle
cell cycle analysis
cell cycle analysis,  photosynthetic prokaryotes
cell cycle kinetics
cell cycle, kinetics
cell fusion
Cell labeling
Cell linker dyes
Cell Migration
cell preparation
Cell Proliferation model
Cell Proliferation Model, Celldiv.
Cell Proliferation Model, Celldiv.equ
Cell Proliferation Model, Complex.Mod
Cell Proliferation Model, Demogr.equ
Cell Proliferation Model, Info List
Cell Proliferation Model, Inside Out
Cell Proliferation Model, model list
Cell Proliferation Model, Outside In
Cell Proliferation Model, range
Cell Proliferation Model, scaling
Cell Proliferation Model, Simple.Mod
Cell Proliferation Wizard, edit model parameter
cell sorter
Cell staining
Cell staining, intracellular
cell staining, intracellular with cell surface
cell staining, intracellular with DNA
cell suspension
cell trackers
cell tracking
Cell tracking, 51Cr release
Cell tracking, activated macrophages
Cell tracking, ADCC
Cell tracking, apoptosis
Cell tracking, apoptotic bodies
Cell tracking, bacteria
Cell tracking, Cell Census Plus tm System
Cell tracking, cell proliferation
Cell tracking, conjugates
Cell tracking, CTL
Cell tracking, cytostasis
Cell tracking, cytotoxicity
Cell tracking, Differentiation
Cell tracking, dye aggregates
Cell tracking, dye dilution
Cell tracking, effector 
Cell tracking, effector or target cells
Cell tracking, general cell labeling 
Cell tracking, general cell labeling- homogeneous staining
Cell tracking, general cell labeling- lymphoid organs
Cell tracking, general cell labeling-adherent cells 
Cell tracking, general cell labeling-PKH dye loading
Cell tracking, homing
Cell tracking, immunotherapy
Cell tracking, in vivo 
Cell tracking, in vivo labeling
Cell tracking, lysis
Cell tracking, membrane retention properties
Cell tracking, migration
Cell tracking, neutrophils
Cell tracking, NK-mediated cytolysis
Cell tracking, phaocytosis
Cell tracking, PKH2
Cell tracking, PKH26
Cell tracking, PKH67
Cell tracking, Proliferation
Cell tracking, propidium iodide
Cell tracking, red cells
Cell tracking, resident peritoneal macrophages
Cell tracking, spectral properties
Cell tracking, tumor cells
Cell tracking, viability
cell walls
cell, concentration
cell, number
CellProbe reagents
cells, circulating
cells, viable
cellular control 
cellulase "Onosuka" R10
Centers for Disease Control (CDC)
centrifugal cytology
centrifugation
charge phase
chicken red blood cells
chimeras
chlorophyll autofluorescence
chloroplast number
chloroquine
Chromomycin A3
chromosome counting
Chromosome isolation buffer
chromosome paints
CICERO
clamp
Cleaning, stream chamber
Cleaning, voltage plates
Cloning
cluster analysis
coefficient of variation (CV, CV%)
coherence
Coherent
Coherent CR599
coincidence
Commercial suppliers
compensation
compensation, cross laser
compensation, fluorescence
compensation, interbeam
compensation, on-line software 
complement elimination
complement, lysis
computer programs
concentrator
conjugation chemistry
conjugations
control, isotype
controls
controls, biological
core facility
Core facility, administration guidelines
Core facility, finance costs
Core facility, general considerations
Core facility, room size
Core facility, set up
Core facility, utilities
Core facility-environmental requirements
core manager
core stream
cost structures
costs
cotransfection
co-transfection vector
co-transfection vector, pMACS Kk 
Coulter Counter
Coulter XL
CPW13M
CPW21S
crosslinking reagent
CY7
cyanobacteria
Cytocentrifuge
cytoenzymology
cytokines
Cytomation, Inc.
cytometer, laser scanning
cytoplasmic domain, truncated
cytosolic rise
Cytospin
cytotoxicity
cytotoxicity assay
DAPI
dark field
DARPA
data acquisition systems
data acquisition, high speed
data analysis
data backup
data collection
data file, standard
data management
data management, network
Data Network, LAN connection
data plot histogram 3D
data plot, contour
data plot, density
data plot, dot
data plot, histogram
data plot, kinetic
data plot, overlay
data transfer
data transfer, direct cable
data, archive
data, instrument setup
data, ownership
data, processed
data, propriety value
data, raw
data, storage
data, transfer
data, user profile
dead cell discrimination
dead cells
decontamination
denaturing solution
dendritic cells
depletion of cellular ATP
desferrioxamine mesylate (DFOM)
detector FL1
detector FL2
detector FL3
detector FL4
detectors
detergents
dextran
dextran acidification
dichroic
dicyanohydroquinone (DCH)
digital logarithmic conversion
digitization
dimethylpolysiloxane (DMPS)
discriminator, lower and upper limit
disinfectants
disk format compatibility
dispersion, biochemical
dispersion, mechanical
disposable filter, Celltrics
DNA analysis
DNA cell cycle
DNA content
DNA fragmentation
DNA shuffling
DNA transfection
DNA yeast
DNA, absolute amounts
DNA, amount estimation error
DNA, quantification
DNA/RNA Content
Dnase
doublet discrimination
drop breakoff
drop delay
drop drive
drop drive frequency
droplet
droplet breakoff
droplet formation
droplet/event profiling
drug delivery
drug selection
dual color analysis using red laser
E. Coli lacZ
EBFP
EGFP
Electrical- current phasing
Electrical- dedicated circuits
Electrical- UPS
Electrical- voltage regulator
electronics, front end
electroporation
emission filters
encapsulation
endocytic vesicles
endocytic vesicles, clamped
endoreduplication
endosomes, early
endosomes, late
endosomes, recycling
enzyme activity
enzyme treatment
EPICS Elite ESP
epi-illumination
Eppendorf tube
esterase
Ethanol
ethidium bromide
eukaryotes
excitation
EXPO software
F(ab')2 fragments
F/P ratio
FACS-Gal
fata files, storage
Fc-receptors
FCS file format
Fetal liver
Feulgen microdensitometry
file server
file structure, FSC
file transfer protocol (ftp)
files, list mode
files,histogram
filter, excitation
Filter, Gelman
Filter, Millipore
Filter, Whatman
filtering, spatial 
filters, band pass
filters, interference
filters, long pass
filters, optical
filters, short pass
FITC
fixation
fixation, 7AAD
fixation, alcohol
fixation, aldehydes
fixation, DMSO
fixation, ethanol
fixation, formaldehyde
fixation, glutaraldehyde
fixation, glyoxal
flicker
flow cell, Sort Sense tm
flow cytometer
flow cytometer, performance
flow cytometer, precision
flow cytometer, sensitivity
flow cytometry
flow cytometry, high speed
flow cytometry, history
flow karyotype
flow rate
flow rate, calibration
flow rate, sample
flow speed
fluidics
fluid-phase endocytosis
fluorescein 
fluorescein di-b-galactopyroanoside (FDG)
fluorescence
fluorescence compensation
Fluorescence In Situ Hybridization (FISH)
fluorescence lifetime spectroscopy
fluorescence pulse height
fluorescence quenching
Fluorescent Proteins Newsgroup
fluorgenic enzyme substrates
fluorochromes
Fluorospheres, antibody conjugated
Fluorospheres, antibody labeling
Fluorospheres, APC
Fluorospheres, cells staining
Fluorospheres, conjugation
Fluorospheres, light yellow
Fluorospheres,Sky Blue
formaldehyde
formaldehyde solution, buffered
forward scatter
funding sources
gain
gating
G-C%
gel microdrop
gene
gene therapy
gene transfer, efficiency
General membrane labeling, immunogenicity
General membrane labeling, membrane retention
General membrane labeling, migration
General membrane labeling, trouble shooting
genes, cytotoxic
genes, growth inhibitory
genetic uniformity
GFP chromophore
GFP mutant, P4-3
GFP, cycle 3 variant
GFP, energy transfer reaction
GFP, extinction coeffiecient
GFP, fusions
GFP, red-shifted 
GFP, spectra
GFP, structure
GFP, troubleshooting
GFP, variants
GFP, wild type (wtGFP)
GFP,extinction coefficient
GFPuv
glass slide, cleaning
glass slide, coating
glutaraldehyde
glycoprotein efflux pump (PGP)
Gordon Gould
green fluorescent protein (GFP)
growth assays
growth inhibition
guard cells, size
haploid
Hematopoetic stem cells (HSC)
Hematopoetic stem cells, fetal liver
Hepatitis viruses
Hepes buffer
Herzenberg, Leonard
high potassium buffer
high speed applications
histogram comparison
histogram marker
histogram, cloud
histogram, single parameter 
histogram, two parameter 
Hoechst 33258
Hoechst 33342
HPRT gene
HS294T cells
human genome mapping
Human Immunodeficiency Viruses (HIV-1,-2)
humanized
hybridity
hybridization, in situ
hybridization, whole cell
hybridoma, cells
HyperSort tm
hypocellular specimens
hypotonic shock
HYTK gene
illumination, critical
illumination,Koehler
image forming optics
immunization
immunofluorescence, 5 color 
Immunofluorescence, 6 color
immunofluorescence, multicolor
In Situ Hybridization
INDO-1
infectious agents
infectious disease hazards
inhalation hazard
institutional support
instrument drift
instrumentation grant
integrator
internal reference
internal standard
Internet
interrogation point
Intestinal alkaline phosphatase (IAP)
intracellular cytokines
intracellular pH (pHi)
intracellular potassium
intracellular staining
inventors
ionomycin
ionophore
ISAC
isometric plot
jet-in-air interogation
Krypton
lab jack
lac-Z gene
lamin B
lamina
laminar flow
laser
laser, air-cooled
laser, argon
Laser, closed loop system
laser, Coherent Enterprise II 621
Laser, cooling requirements
laser, Cyonics
laser, diode
laser, diode-pumped solid state
laser, dye
Laser, heat exchangers
laser, Helium-Cadmium
laser, Helium-Neon
laser, ion
laser, maintenance
laser, noise
laser, output wavelength
laser, safety
laser, UV
laser, water-cooled
Leif centrifugal cytology buckets
libraries, cosmid & phage
light collection
light scatter
limited dilution
lineage marker cocktail
Linscott's Directory
listmode data acquistion
log amp
logarithmic amplification
Los Alamos
lysis buffer
lysosomes
MACS magnetic beads
magnetic beads
marine samples
marine samples, preservation
Mayers' albumin fixative
mean fluorescence intensity
media, gradient
Membrane Dyes
Membrane dyes, bacteria
Membrane dyes, dye aggregate
Membrane dyes, endothelial cells
Membrane dyes, epithelial cells
Membrane dyes, GL diluent
Membrane dyes, hematopoetic cells
Membrane dyes, Immune cells
Membrane dyes, In Vivo labeling
Membrane Dyes, intercalating
Membrane Dyes, lipophilic
Membrane dyes, lymphocytes
Membrane dyes, PCL diluent
Membrane dyes, Peritoneal Macrophages
Membrane dyes, phagocytic cell labeling
Membrane dyes, protoplasts
Membrane dyes, saponin
Membrane dyes, self-quenching
Membrane dyes, spectral characteristics
Membrane dyes, splenocytes
Membrane dyes, stem cells
Membrane dyes, tumor cells
Membrane dyes, viruses
membrane integrity
membrane proteins
mercury gas
metabolites
methyamine
MFI
microbeads
microdrop, in situ hybridization (MISH)
microdrops, singly occupied
microscope slide scanning
microtiter plates
mirrors, dichroic
mirrors, dichroic
mixed-gas
mixing, sample and sheath fluids
Mock Transfection Control 
modeling programs
ModFit G1 scaling
ModFit v5.2
MoFlo r
monensin
monoclonal antibodies
monolayer slide
morphological analysis
multicolor
multicolor analysis
multi-laser benches
multi-line UV
multi-parameter analysis
multiparameter applications
multi-wavelength mode
mutagens
Myeloma cells
National Committee for Laboratory Standards (NCCLS)
Nd:YAG
necrosis
neomycin
nerve growth factor receptor (NGFR)
network cabling
network manager
network, data flow
network, security
network, transfer compatibility
nigericin
NIH programs
NIST
noise
normalization
nozzle
nuclear matrix (NM)
nuclear matrix protein (NMP), cleavage and degradation
nuclear mitotic apparatus protein (NuMA)
nuclei isolation
nuclei, preparation
nucleoporin
numeric aperature (NA)
nylon mesh
obscuration bar
observation point
occupational hazards
oil immersion optics
oligotrophic waters
oligotrophic waters, surface sample
Optical Configuration, (7AAD)
Optical configuration, 2 laser ( 7 parameter, 5 color)
Optical configuration, 3 color
Optical configuration, 3 laser ( 7 parameter, 5 color)
Optical Configuration, 6 color
optical filters
optics
optics, collection
Orthogonal configuration
orthogonal scatter
oxidative burst
packaging cell line
Pancreas digestion
panning, antibody
panning, lectin
paraformaldehyde
parallel processing
paramagnetic beads
parameter, ratio of
parameter, time
Parameters
Parameters, extrinsic
Parameters, functional
Parameters, intrinsic
Parameters, structural
parsley
Partec 
Partec CA-III
Partec flow cytometer
pathogens
peak and holds
peak detector
peak mode
peak resolution
peanut agglutinin
PE-APC
Pectolyase Y23
PE-CY-5
pepsin
peritoneal lavage
permeabilization
Personal protective equipment
personnel management
phase-sensitive detection
phenlyethyl-thio-galactoside (PETG)
phenotypic analysis
phorbol myristate acetate (PMA)
Phosphate buffered saline (PBS)
phosphatidylserine
photomultiplier tube (PMT)
photon
phytoplankton
PI linear signal
PI peak signal
picoeukaryotes
PicoGreen
picoplankton
picoplankton, photosynthetic
pigments
PKH dyes
PKH dyes, Cell Census Plus tm System
PKH dyes, Diluent B
PKH dyes, general membrane labeling
PKH dyes, head group
PKH dyes, hydrocarbon tails
PKH dyes, in vivo labeling
PKH dyes, in vivo labeling - peritoneal macrophages
PKH dyes, in vivo labeling - PKH2 labeling321
PKH dyes, in vivo labeling - recruited macrophages
PKH dyes, in vivo labeling - resident macrophages
PKH dyes, inflammation
PKH dyes, internal count standard
PKH dyes, Internal Count Standards
PKH dyes, lipopolysaccharide (LPS)
PKH dyes, monocytes
PKH dyes, neutrophils
PKH dyes, particle ingestion
PKH dyes, peripheral blood lymphocytes
PKH dyes, phagocytic cells
PKH dyes, PKH Microbeads
PKH dyes, PKH2
PKH dyes, PKH26
PKH dyes, PKH26 in lymphocytes
PKH dyes, PKH-26 Microbeads
PKH dyes, PKH-26 Microbeads- absolute cell counts
PKH dyes, PKH67
PKH dyes, Reference Microbeads
PKH dyes, reference Microbeads- Absolute cell counts
PKH dyes, Standard Cell Dilution Assay
PKH dyes-PKH26
PKH dyes-PKH26, labeled erythrocytes
PKH26
PKH26, detection of donor cells
PKH26, fluorescence
plama tube
Plant DNA
plaque cytogram assays
plasma tube
plastids
platelets
ploidy
ploidy level
p-MACS 4 vector 
pMACS vector
poisson statistics
pollen size
polyclonal antibody
poly-lysine
polyvinylpryrrolidone (PVP-40)
Post-exposure prophylaxis
pre-enrichment
pre-enrichment, lin(-) fetal liver
prisms
probes, oligonucleotide
Prochlorococcus
prochlorophytes
progenitors, long-term self-renewing
prokaryote
proliferating cells
Proliferation wizard, daughter generation
Proliferation wizard, deconvolution analysis
Proliferation wizard, Generations
Proliferation wizard, Parent
Proliferation wizard, parent generation
propidium iodide
proteases
protoplasts
publications
pulse processing
pulse shape analysis
pulse width measurement
Pyronin Y
quality control (QC)
quality control log
quenching
rare cells
ratio fluorescence emission
ratio fluorescence value
ratio signal
ratiometric analysis
RBC lysing buffer
red blood cell lysis
red blood cells
reporter genes
resonator
revenues
Rhodamine 6G
risk of exposure
Rosa fedtschenkoana
rose callus
rose leaves
S65T
safety
sample block, special sorting
sample core
sample handling
sample preparation
Sample preparation, high speed sorting
sample processing
samples, natural
samples, unfixed
samples, unwashed
saponin
saturation
SBIR
Sca-1
scatter plot
scatter, forward 
scatter, side 
secondary control
secretion-capture assay
selectable markers
service contract
settings, channel
settings, voltage 
sheath
sheath flow
sheath fluid, sea water
signal intensity
signal-to-noise
signal-to-noise ratio
Single cell suspension
smear, conventional
SNARF-1 AM
software
sort decision
sort envelope
sort, rare cell
sorting
Sorting unfixed cells, guidelines
sorting, high speed 
sorting, purity
sorting, recovery
specific binding
specificity
spectral overlap
Spectra-Physics
splenic T-cells
splenocyte, isolation
spreadsheet
stability
staining
staining, direct
staining, indirect
staining, non-specific
standard deviation
standards, external
standards, internal
standards, internal, plant vs animal
starch
statistical analysis, quadrant
sterile filtration
Sterilization, cell sorter- hydrogen peroxide
Sterilization, cell sorter- sodium hypochlorite
Sterilization, cell sorter-bleach
Sterilization, external components
STIR
stream velocity
streptavidin
super-para-magnetic bead
surface antigens
survival/lifespan
SYBRtm Green-I
Synechococcus
syringe, threaded plunger
tandem conjugates
taxonomy
T-cell subsets
TEM00
temperature
thapsigargan
threshold
Thy1.1
thymocytes
time
timed pregnancy
timing circuits
tissue chopping
titration
titration curve
TO-PRO-1
TO-PRO-3
TOTO-1
toxins
tracking
Tracking dye
transduction
Transfection, Calcium phosphate
Transfection, electroporation
Transfection, Liposome-mediated
Transfection, stable
Transfection, transient
transfections
transferrin
transferrin acidification
Transfluospheres
transgenic mice
trigger signal
TurboSort r
Tween-20
Uniphase
Universal precautions
User fees
User groups
user training
uv excitation
vacuoles
vector
vector, dual promoter
vector, retroviral
viability
Waste disposal
white light
world wide web
xenon gas
yellow fluorescent protein
YO-PRO-1
YOYO-1
Zyn-linkers